This study was conducted in the fields of the Department of Animal Production, College of Agriculture, University of Kirkuk, for the period from 7/24/2023 until 10/1/2023, with the aim of adding different levels of chicken egg shell powder to diet of Lohman laying hens and its effect on the blood characteristics. 100 hens with 28-weeks-old were used, and divided randomly into five treatments, 20 birds for each treatment (T1=0% egg shell powder, T2=25% egg shell powder, T3=50% egg shell powder, T4=75% eggshell powder, T5=100% eggshell powder). Fresh blood samples were withdrawn from the wing vein from the chicken and placed in test tubes devoid of anticoagulants and left for 6 hours to analyse some blood serum characteristics (Calcium, phosphor, LDL, HDL, AST, ALT, total protein, uric acid, and total cholesterol). SAS version 4.9 and the CRD method were used to analyse data, Duncan's multi-range test was employed to determine how much the means of the coefficients for each attribute under study varied from one another. The present study indicates, using of eggshell powder in the diet of the laying hens decrease the AST liver enzyme, and the total cholesterol on the blood, and did not effect on the other blood parameters.