In veterinary research facilities, antimicrobial disinfectants and antiseptics are thought to be the primary line of protection against any harmful bacteria on various inanimate surfaces to aid in the prevention of healthcare association infections (HAIs). The study goals were to estimate the prevalence rate of bacterial pathogens in the surrounding environment of veterinary research facilities, assess the antimicrobial pattern of newly formulated disinfectants (Sporocide Glu®, Cox killer®, and Klorsept 25®) and two antiseptics (ethyl alcohol 70% (w/v) and chlorohexidine HCL (125mg/100ml)) against all isolated bacterial pathogens, and establish a control strategy for preventing the spread of bacterial contaminants to researchers and the lab environment. A total of 236 swab samples were taken from the lab environment (n = 149), equipment (n = 57), and lab researchers (n = 30) in the seven research veterinary laboratories. The sensitivity profile of thirty strains of bacterial isolates to various disinfectants and antiseptics was evaluated. Results, the most common bacterial isolates in all lab environmental samples were E. coli and S. aureus (35.5% each). At 0.7 and 1.0% concentrations, SG® disinfectant exhibits 100% biocidal action against S. aureus, CNS, Klebsiella spp., and Pseudomonas spp. Oppositely, hydrogen peroxide (H2O2) was 100% effective against all bacterial isolates, with the exception of S. aureus, which was 83.3% effective at the highest dose tested (6.0%). In conclusion, Sporocide Glu® (1%), Klorsept 25® (0.4 mg/l) disinfectants, and chlorohexidine HCL (125 mg/100 ml) antiseptics proved their bactericidal action (100%) against all bacterial isolates in the surrounding environment of labs.