The present study was designed to demonstrate the effect of Graviola (Annona muricata) on some physiological parameters and the reproductive efficiency of male rats exposed to oxidative stress.  A total of 48 Wister albino rats were distributed among four groups, each group containing 12 rats, control group, A group was administered H₂O₂ at a concentration of 0.5% for 2 months), a group was given H₂O₂ with Graviola 100 mg/kg/day for 2 months) and a group was dosed with Graviola 100 mg/kg/day for 2 months). The results of this study showed a notable decrease in sperm count, the percentage of sperm viability, the antioxidant activity (AOA), the level of sex hormone (testosterone, follicular stimulating hormone and luteinizing hormone), glutathione concentration, Superoxide Dismutase enzyme activity, germinal epithelium height, and seminiferous tubules diameter in H₂O₂ group. Likewise, there were significant increase in MDA, percentage of sperm abnormalities and dead sperm, in the H₂O₂ group compared to the control group. Though, when H₂O₂ is given in combination with Graviola, there was a significant improvement. This combined treatment led to a significant increase in sperm count, sperm viability, sex hormone levels, glutathione concentration, SOD activity, antioxidant activity, height of germinal epithelium, and the seminiferous tubules diameters. At the same time, there is a significant decrease in MDA, the percentage of sperm abnormalities and dead spermatozoa. In conclusion, H₂O₂ treated with Graviola efficiently restores antioxidant parameters, sperm count, and sperm viability to normal values. This research proposes that Graviola provide good benefits in enhancing the reproductive performance of male rats.