Metabolomics is the study of metabolites, small molecule intermediates, and metabolites. We report the metabolomics in the plasma of mice administered SNL PGF2α and PMSG during synchronized and super ovulated protocols. Groups of 3  female Albino mice were each treated first group PGF2α 14.7 µg\kg BW. and PMSG 8.3 IU\kg BW.  while second group SNL PGF2α 6.6 µg\kg BW. and PMSG 8.3 IU\kg BW.  and, there group.  The control group received no treatment. Metabolites in plasma were extracted with methanol-chloroform-water and identified by gas chromatography-mass spectrometry (GC Mass). The remarkable markers of candidates groups of plasma provide 221 total number in conventional were 76, in control 75 metabolomics, while in SNL 72 metabolomics when comparing between the groups in the conventional  group higher than other groups.