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359164

Toxoplasma antibodies, Toxoplasma-DNA and Antibodies of HCV and HIV in Patients with Hemodialysis in Beni-Suef, Egypt

Article

Last updated: 23 Dec 2024

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Subjects

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Tags

Veterinary Parasitology & Microbiology

Abstract

Globally, Toxoplasma gondii (T. gondii) infections are frequent in both humans and animals, and toxoplasmosis is among the most prevalent parasitic zoonoses.  Hemodialysis (HD) is a potentiating factor for impaired cellular immunity, making patients more susceptible to the opportunistic pathogen Toxoplasma gondii and blood-transmitted viruses. To identify the sero-occurrence of Toxoplasmosis, HCV and HIV and the molecular-occurrence of Toxoplasma within the sera of patients undergoing HD. A single serum sample was collected from 75 HD patients. Serum samples were searched for anti-Toxoplasma IgG and IgM, HCV and HIV antibodies (Abs) using ELISA and Toxoplasma DNA using PCR assay. The results of this study indicated that Toxoplasma was detected by IgG, IgM and PCR (61.3%, 22.7%, and 1.3%, respectively). HCV Abs was detected in 14.6% of cases, co-infection with Toxoplasma (Abs) in 8 cases (10.6%), while HIV Ab wasn't detected in any patients by ELISA. This investigation concluded that seropositive Toxoplasma and HCV are frequent in HD patients with a very low prevalence of Toxoplasma DNA and regular checks for Toxoplasma and HCV seroconversion is desirable in these patients. A two-steps diagnostic immune-molecular approach in one single serum sample for detection of Toxoplasma-DNA in seropositive patients can rule out acute or active toxoplasmosis and avoid unnecessary treatment.

DOI

10.21608/ejvs.2024.284997.2034

Keywords

toxoplasma gondii, Ig G, Ig M, PCR, ELISA, HCV, HIV

Authors

First Name

Hala

Last Name

El-Askaryˡ

MiddleName

M.

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

Email

halaelaskary@hotmail.com

City

-

Orcid

-

First Name

Ayman

Last Name

El-Badry

MiddleName

A

Affiliation

Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

aymanbb@hotmail.com

City

-

Orcid

-

First Name

Raafat

Last Name

Shaapan

MiddleName

Mohamed

Affiliation

Dept. of Zoonotic Diseases, Veterinary Research Division, National Research Centre

Email

rmshaapan2005@yahoo.com

City

Cairo

Orcid

0000-0002-2620-4189

First Name

Mousa

Last Name

Ismail

MiddleName

A. M.

Affiliation

4Medical Parasitology, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Egypt

Email

mousa.ismail@kasralainy.edu.eg

City

-

Orcid

-

First Name

Marwa

Last Name

Ghieth

MiddleName

A.

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

marwaghieth@yahoo.com

City

-

Orcid

-

First Name

Mostafa

Last Name

S. Sheemy

MiddleName

-

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Y. Ismaeel

MiddleName

-

Affiliation

Internal Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

Email

-

City

-

Orcid

-

First Name

Shimaa

Last Name

A Senosy

MiddleName

-

Affiliation

Public Health and community Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

-

City

-

Orcid

-

First Name

Mona

Last Name

I. Ali

MiddleName

-

Affiliation

Departments of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

Email

-

City

-

Orcid

-

Volume

56

Article Issue

6

Related Issue

48121

Issue Date

2025-06-01

Receive Date

2024-04-24

Publish Date

2025-06-01

Page Start

1,239

Page End

1,246

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 6_Pages 1239-1246.pdf

PDF . 1.0MB

Link

https://ejvs.journals.ekb.eg/article_359164.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=359164

Order

359,164

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Toxoplasma antibodies, Toxoplasma-DNA and Antibodies of HCV and HIV in Patients with Hemodialysis in Beni-Suef, Egypt

Details

Type

Article

Created At

23 Dec 2024