This study was conducted to prove the physiological impacts of different concentrations of aqueous extract of ginger on some biochemical traits in mature ewes to find the best concentration to investigate the changes that happened in the physiological parameters of the animal. The study investigated the impact of aqueous extraction of ginger tubers at concentrations of 200, 300, and 400 mg/kg of body weight and depended on these treatments for 20 ewes for two months. The gradual increase in the approved concentrations of the aqueous extract of ginger tubers has also led to a gradual significant increase (p≤0.05) in the approved biochemical parameters, such as a decrease in blood glucose, an increase in total protein levels and blood albumin concentration, and a decrease in the level of AST and ALT liver enzymes. The results of the current study proved that treating of adult ewes by aqueous extracts of ginger tubers in different concentrations showed gradual important improvement and stability in vital biophysiological traits.