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357678

Genetic Differences and Pathogenicity of Chicken Anemia Virus Strains in Broiler’s Baby Chicks

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Avian and aquatic Diseases

Abstract

This study  aims to molecular characterization of the Chicken Anemia Virus (CAV) in young broilers in the Giza governorate, Egypt in 2021, assess the genetic diversity among the proliferating viruses, and vaccine strains, and evaluate their pathogenicity to improve viral management. Viral protein 2 (VP2) gene was amplified and subsequently conducted molecular-level characterization. In five out of the ten flocks sampled CAV was detected. Furthermore, the examination of the phylogenetic connections performed according to the sequences of the VP2 gene demonstrated the classification of the observed field strains within genogroups I, II, and IIIb. In silico analysis was performed on Cux-1 and the detected five strains to investigate the secondary structures of VP2 proteins. The findings indicated a significant resemblance in the distribution and quantity of α-helical and β-strand topologies. To assess the pathogenicity of the virus, two isolates from genotype III (MZ574096.1) and genotype II (MZ477000.1) were administered intramuscularly to sets of day-old, specific pathogen-free chicks. At 28 days following inoculation, the Red Blood Corpuscle count (X106µL) was (1.4 ±0.39 and 1.8± 0.12).

DOI

10.21608/ejvs.2024.256791.1736

Keywords

CAV, Genetic Characterization, VP2, Young chicken

Authors

First Name

Hanaa

Last Name

Elsamadony

MiddleName

-

Affiliation

Poultry Diseases Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza, Egypt

Email

hanaaelsamadony@gmail.com

City

-

Orcid

0000-0002-9770-9854

First Name

Hoda

Last Name

Mekky

MiddleName

M

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Giza, Egypt.

Email

hmekky335@gmail.com

City

Giza, Egypt

Orcid

0000-0002-9808-7065

First Name

Hanaa

Last Name

Fedawy

MiddleName

-

Affiliation

Poultry Diseases Department, Veterinary Research Institute, National Research Centre, P.O. 12622, Giza, Egypt

Email

hanaafedawy@yahoo.com

City

-

Orcid

0000-0001-7253-667

First Name

Dalia

Last Name

Sedeek

MiddleName

-

Affiliation

Poultry Diseases Department, Veterinary Research Institute, National Research Centre, P.O. 12622, Giza, Egypt

Email

daliamsedeek@gmail.com

City

-

Orcid

0000-0001-8271-0089

First Name

Ahmed

Last Name

EL-Shemy

MiddleName

-

Affiliation

Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12622, Egypt.

Email

dr.ahmedelshemy@gmail.com

City

Cairo

Orcid

0000-0001-9578-5952

First Name

Aalaa

Last Name

Saad

MiddleName

-

Affiliation

4) Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. 12611, Giza, Egypt

Email

alaa.samir87@yahoo.com

City

Giza

Orcid

0000-0003-4740-4419

Volume

56

Article Issue

4

Related Issue

47682

Issue Date

2025-04-01

Receive Date

2023-12-20

Publish Date

2025-04-01

Page Start

851

Page End

861

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 4_Pages 851-861.pdf

PDF . 1.4MB

Link

https://ejvs.journals.ekb.eg/article_357678.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=357678

Order

357,678

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Genetic Differences and Pathogenicity of Chicken Anemia Virus Strains in Broiler’s Baby Chicks

Details

Type

Article

Created At

23 Dec 2024