Avian mycoplasmosis is one of the most significant financial threats affecting the global poultry business so, convenient approach for detecting etiological agents; Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS)to characterize the circulating field strains and determine their antimicrobial susceptibility profiles. A study conducted between 2017 to 2022 in Kafrelshekh, Sharkia and Dakahlia, governorates in Egypt on different avian sectors, Twelve MG and four MS isolates were identified by culture and confirmed by PCR. Partial sequence of mgc2 gene of two MG isolates from turkey (MGB.T.) and (MGW.T.) was submitted on gene bank under accession no.OM632677.and OM632678 respectively and revealed that there are two different MG circulating strains in turkey, for determining the antimicrobial susceptibility profiles , ten MG, one MS isolates from different avian sector were tested against eight antimicrobials for determination of lowest MIC, three antibacterials, tiamulin (0.009μg/ml), tilmicosin (0.039μg/ml) and tylosin (0.019μg/ml), offered the lowest MICs of all efficient medications .MS isolate was sensitive for the eight antimicrobials, most MG isolates were sensitive to ciprofloxacin and streptomycin while Lincospectin has intermediate effect. Isolate MG11from baladi chicks was completely resistant to all antimicrobial. The two MG isolates from turkey were resistant to (erythromycin and tylosin), so, further investigation for macrolides resistant genes detection is required. The two MG isolates from turkey were resistant to (erythromycin and tylosin), so, further investigation for macrolides resistant genes detection is required. In conclusion, the present study confirms the presence of MG infection in turkey and chicken flocks of Sharkia and Dakahlia governorates.