The study investigates the effect of a month's treatment with hydrocortisone acetate on rat fertility and sperm activity. The study included 24 adult male rats divided into 3 groups. The first group (control) received DW, while the second received 2.5 mg/kg hydrocortisone. The third group received 5 mg/kg hydrocortisone. The injection period lasted 30 days, with an injection rate of 3 times weekly. The animals were anesthetized at the end of the treatment period. The sperm motility was calculated. Several readings taken and each reading was the same estimate of the sperm movement rate. The sperm concentration was calculated, and the live was classified as dead or mutilated. Blood was also taken to examine the testosterone concentration. The results showed that giving hydrocortisone had a dose-dependent effect on the number of sperm and their forward movement compared to the control group. The results also revealed a drop in testosterone levels in rats treated with low and high dosages of hydrocortisone. The findings also indicated an increase in the number of dead and malformed sperm. The study concluded that hydrocortisone at the levels used in the study has a direct negative effect on sperm and their activity, which is reflected in the animal's fertility. The present study concludes that hydrocortisone, even at modest doses, may have abnormal effects on spermatogenesis in the testes. In this present study, we recommend an extensive study on the impact of hydrocortisone drug to investigate the causes of infection and extend its infertility.