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354498

Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Animal Reproduction & Artificial Insemination

Abstract

This study investigates the consequence of adding platelet-rich plasma (PRP) on the post-thawed quality and in vitro fertility of buffalo bull semen. Fifty-four ejaculates from nine buffalo bulls were collected, and every ejaculate was then dispensed into 5 equal parts, diluted with Optixcell extender supplemented with five [0, 5, 10, 15, and 20] proportions of PRP followed by freezing using the usual protocol. Post-thaw sperm motility, sperm viability, the integrity (%) of the plasma membrane, acrosome, DNA, and mitochondrial membrane potential (%) were evaluated. Total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) content as well as in vitro fertilization (IVF) were also assessed. The obtained results revealed that the 15 % PRP-supplemented extender improved sperm viability, progressive motility, and integrity of the plasma membrane. All 5 %, 10 %, and 15 % PRP treatments greatly reduced (P < 0.05) MDA and increased GPx and SOD activities. The cleavage rate was greatly increased (P < 0.05) following IVF with 15 % PRP-supplemented spermatozoa. In conclusion, the incorporation of 15 % PRP into the freezing extender is recommended to ameliorate freezing-thawing damage on sperm cell membranes and increase the in vitro fertility of buffalo bull spermatozoa.

DOI

10.21608/ejvs.2024.254249.1712

Keywords

Buffalo, frozen-thawed, Mitochondrial membrane, in vitro fertilization, PRP

Authors

First Name

Essam

Last Name

Almadaly

MiddleName

A.

Affiliation

Kafrelsheikh University, Faculty of Veterinary Medicine, Department of Theriogenology, El-Geish Street, Kafrelsheikh 33516, Egypt

Email

dr_essam_2005@yahoo.com

City

Kafrelsheikh

Orcid

0000-0003-3598-8090

First Name

Ibrahim

Last Name

Mansour

MiddleName

-

Affiliation

2 Production Sector, Agricultural Research Center, Ministry of Agriculture, 33516 Kafrelsheikh, Egypt.

Email

ibrahimmansour44@yahoo.com

City

-

Orcid

-

First Name

Ferial

Last Name

Sahwan

MiddleName

-

Affiliation

Alexandria University, Faculty of Veterinary Medicine, Animal Breeding and Production, Animal Husbandry and Wealth Development Department, Egypt.

Email

ferial66@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

M. Shehabeldin

MiddleName

-

Affiliation

Biotechnology Research Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

Email

ahmedshahabvet@yahoo.com

City

-

Orcid

-

First Name

Ismail

Last Name

El-Kon

MiddleName

-

Affiliation

Kafrelsheikh University, Faculty of Veterinary Medicine, Department of Theriogenology, El-Geish Street, Kafrelsheikh 33516, Egypt.

Email

ismailelkhon775@yahoo.com

City

-

Orcid

-

First Name

Abdelaziz

Last Name

Sakr

MiddleName

-

Affiliation

Animal Production Research Institute (APRI), Agriculture Research Center, Ministry of Agriculture, Dokki, 12618 Giza, Egypt

Email

abdosakragri@yahoo.com

City

-

Orcid

-

First Name

Wael

Last Name

Eldomany

MiddleName

-

Affiliation

Mansoura University, Faculty of Veterinary Medicine, Department of Theriogenology, Mansoura, Egypt.

Email

waeleldomanyvetman@yahoo.com

City

-

Orcid

-

First Name

Adel

Last Name

A. Ramoun

MiddleName

-

Affiliation

Kafrelsheikh University, Faculty of Veterinary Medicine, Department of Theriogenology, El-Geish Street, Kafrelsheikh 33516, Egypt

Email

-

City

-

Orcid

-

Volume

56

Article Issue

3

Related Issue

47550

Issue Date

2025-03-01

Receive Date

2024-01-06

Publish Date

2024-03-01

Page Start

617

Page End

626

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 3_Pages 617-626.pdf

PDF . 1.3MB

Link

https://ejvs.journals.ekb.eg/article_354498.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=354498

Order

354,498

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Details

Type

Article

Created At

23 Dec 2024