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354307

Efficacy of Astragalus Polysaccharide versus Enrofloxacin on Campylobacter Jejuni Colonization in Broiler Chickens

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Pharmacology & Toxicology

Abstract

Campylobacteriosis is the world's most common zoonotic disease, affecting broiler chickens. A study was conducted to assess the effectiveness of Astragalus polysaccharides (APS) versus the antibiotic enrofloxacin (ENR) in controlling Campylobacter jejuni. A total of 160 Hubbard broiler chicks ( 1 day old) were randomly divided into eight groups: healthy chicks (H), healthy chicks given ENR (HA), APS (HP), both (HAP), experimentally infected chicks (I), infected chicks given ENR (IA), APS (IP), and both (IAP). On days 18 and 35, the Campylobacter load was assessed in the cecal contents. The study also evaluated serum immune parameters at various intervals post-infection. Three chickens were slaughtered after the last oral dose of ENR to determine ENR and its metabolite ciprofloxacin (CIP) residues by HPLC in different tissues. The experiment involved estimating the antioxidants of breast muscles, as well as their physico-chemical characteristics, along with measuring serum liver enzymes. The treated groups showed a significant decrease in bacterial count, with no growth observed in the IA and IAP groups after 35 days. ENR and APS significantly reduced serum interleukin-6 (IL-6) and nitric oxide (NO) levels in infected groups while increasing lysozyme levels in all experimental groups. APS lowered MDA levels and increased both SOD and GSH levels in breast muscle. Serum ALT and AST activities were higher in I- and IA- groups compared to healthy and APS-administered groups. APS did not affect marker residue concentrations in the HAP group tissues, but increased concentrations in the IAP group tissues without an increase in drug withdrawal time (3 days). These findings suggest that APS is necessary to improve meat quality and immunity, reduce oxidative damage induced by enrofloxacin and infection, and demonstrate anti-Campylobacter action in vivo.

DOI

10.21608/ejvs.2024.270975.1860

Keywords

Enrofloxacin, Campylobacter, Astragalus polysaccharides (APS), withdrawal time, oxidative damage

Authors

First Name

Maha,

Last Name

Abd-Elhafeez

MiddleName

S.

Affiliation

Chemistry, Toxicology and Feed Deficiency Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza 12619, Egypt.

Email

mahasabry86_doctor@hotmail.com

City

Giza

Orcid

0000-0002-5201-0214

First Name

Fatma

Last Name

Amro

MiddleName

H.

Affiliation

Reference laboratory of veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute ( AHRI), Agriculture Research Center (ARC), Giza 12619, Egypt.

Email

fatmaamro_87@hotmail.com

City

Giza

Orcid

0000-0001-9193-8528

First Name

Heba

Last Name

Farhan

MiddleName

E.

Affiliation

Bacteriology Dept., Animal Health Research Institute, Dokki, Giza 12618, Egypt.

Email

dr.hebaemam@yahoo.com

City

egypt

Orcid

0000-0002-5826-5103

First Name

Pierre

Last Name

Mehanny

MiddleName

E.

Affiliation

Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt

Email

pezzat7@gmail.com

City

Egypt

Orcid

0009-0000-3282-757X

First Name

Farida

Last Name

Mohamed

MiddleName

H.

Affiliation

Immunology Dep., Animal Health Research Institute, Agricultural Research Center, Giza 12619, Egypt

Email

farida201444@yahoo.com

City

Egypt

Orcid

0000-0002-6357-5085

First Name

Gomaa

Last Name

Elhaddad

MiddleName

Y.

Affiliation

Biotechnology Dep., Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute Damanhur Branch, 22511 Agriculture Research Center, Egypt.

Email

gomaaelhaddad@gmail.com

City

Egypt

Orcid

0009-0004-0997-5370

First Name

Eman

Last Name

Elkhawaga

MiddleName

-

Affiliation

Food Hygiene Dept., Animal Health Research Institute, Mansoura, Agricultural Research Center, Egypt

Email

emanelkhawaga@yahoo.com

City

Egypt

Orcid

0009-0007-2818-498X

First Name

Amany

Last Name

Abdelnaby

MiddleName

-

Affiliation

Toxicology Dep., Animal Health Research Institute, Banha Branch, 13512, Agricultural Research Center, Egypt,

Email

amany.abdelnaby19@fvtm.bu.edu.eg

City

Egypt

Orcid

0000-0001-9239-5289

First Name

Fady

Last Name

Youssef

MiddleName

Sayed

Affiliation

Department of Pharmacology, Faculty of Veterinary Medicine, Cairo university

Email

fadyalsalhany@cu.edu.eg

City

Giza

Orcid

0009-0008-2159-8837

Volume

56

Article Issue

3

Related Issue

47550

Issue Date

2025-03-01

Receive Date

2024-02-19

Publish Date

2024-03-01

Page Start

543

Page End

557

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 3_Pages 543-557.pdf

PDF . 1.4MB

Link

https://ejvs.journals.ekb.eg/article_354307.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=354307

Order

354,307

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Efficacy of Astragalus Polysaccharide versus Enrofloxacin on Campylobacter Jejuni Colonization in Broiler Chickens

Details

Type

Article

Created At

23 Dec 2024