ManageChatDiscover
354256

Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Animal Reproduction & Artificial Insemination

Abstract

This study aimed to identify the single nucleotide polymorphisms (SNPs) in a partial sequence of the leptin gene and its potential association with the semen quality parameters and mRNA expression levels of the leptin gene in the Egyptian buffalo' bulls. A total of 20 Egyptian buffalo bulls were used in this study. The selected amplicon of the leptin gene was amplified using a specific primer. After that, the SNPs detection and genotyping were performed based on sequence analysis and high-resolution melting (HRM)-PCR. A c.216A>G synonymous SNP (g.14328A>G) was identified in the assessed partial leptin sequence. This SNP showed significant linkages with the semen profile. In this regard, the heterozygous AG genotype exhibited significantly higher progressive motility (PR), sperm concentration, total sperm count, and higher LH and testosterone hormone levels. While the GG genotype showed higher normal morphology form % and functional sperm %. Moreover, AG and GG genotypes displayed significant upregulation of leptin mRNA levels compared to the AA genotype. The g.14328A>G SNP could be considered an effective marker in marker-assisted selection and breeding programs for improving the fertility of Egyptian buffalo bulls. 

DOI

10.21608/ejvs.2024.276609.1921

Keywords

leptin, Egyptian buffalo, SNP, HRM-PCR, fertility

Authors

First Name

Safaa

Last Name

Abdo

MiddleName

E.

Affiliation

Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Email

safaa_2m@yahoo.com

City

-

Orcid

-

First Name

Esraa

Last Name

Abdelghany

MiddleName

S.

Affiliation

Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

Email

esraa.samy@vet.kfs.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Helal

MiddleName

-

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

Email

mayh1167@yahoo.com

City

-

Orcid

-

First Name

A.

Last Name

Sakr

MiddleName

M.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt

Email

abdosakr521@gmail.com

City

-

Orcid

0000-0001-6800-1171

First Name

Mohammed

Last Name

Abu El-Magd

MiddleName

-

Affiliation

Department of Anatomy& Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

mohrizk73@yahoo.com

City

-

Orcid

-

First Name

mohamed

Last Name

Hegazy

MiddleName

-

Affiliation

Department of Biotechnology, Animal Production Research Institute, Egypt

Email

mmgad2120@gmail.com

City

-

Orcid

0000-0001-5085-8302

First Name

Seham

Last Name

EL-Kassas

MiddleName

-

Affiliation

Animal, Poultry and Fish Breeding and Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

Email

seham.elkassas@vet.kfs.edu.eg

City

-

Orcid

0000-0001-8083-6876

Volume

56

Article Issue

3

Related Issue

47550

Issue Date

2025-03-01

Receive Date

2024-03-15

Publish Date

2024-03-01

Page Start

501

Page End

510

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 3_Pages 501-510.pdf

PDF . 1.3MB

Link

https://ejvs.journals.ekb.eg/article_354256.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=354256

Order

354,256

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls

Details

Type

Article

Created At

23 Dec 2024