The study was conducted to know the effect of Biotin and hCG on the reproductive performance of local Iraqi ewes. Twenty ewes of 2-3 years old and weighted 34-48 kg were used during the period from August 2022 to February 2023. The first group (C) serve as control, while T1, T2 and T3 serve as treatments group, T1 were given hCG- COX 100 I.U/ewe injection, T2 biotin 10 mg/ ewe/ daily oral (30 days) and T3 biotin 10 mg/ ewe/ daily oral (30 days) + hCG- COX 100 I.U./ ewe injection. The estrus rate was equal in all treatments, as all ewes showed Estrus. Pregnant ewes and lambs born increased in the (T3), with it equal. The percentage of C, T1, and T2 (Fertility rate, Conception rate, Pregnancy rate and Lambs rate) was equal (80%), with the superiority of T3 with 100%. Gestation period for C, T1, T2 and T3 were (151.5 ± 4.5, 152.5 ± 6.2, 153 ± 5.2 and 153.8 ± 10.2 days) respectively. Litter size is equal in all experimental groups. Barrenness rate increased in all treatments except for the T3, when it was low. It was concluded from the current study that effect of biotin and hCG was beneficial on reproductivel performance.