Beta
351211

Comparable Study of Immunological, Bacteriological, and Molecular Techniques for Detecting Brucellosis in Milk of Reproductively Problematic Cows

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

Brucellosis, a serious animal infectious disease transmitted to human beings, is a major health risk for dairy consumers, especially in developing-countries resulting in dangerous economic and financial problems. Brucellosis diagnosis is critical in order to detect this infection and find a suitable treatment. Accordingly, this study aims to follow up on the accuracy and sensitivity of different diagnostic techniques used in detecting brucellosis in milk (immunological, bacteriological, and molecular techniques). Milk ring test (MRT) and indirect enzyme-linked immunosorbent assay (i-ELISA) are among the used techniques where positive brucellosis was detected in milk samples with total percentages of 34.91% and 33.96%, respectively. The recorded relative-sensitivity for MRT and i-ELISA were 93.55% and 96.77%, respectively, while their relative-specificities were 89.33% and 92%, respectively. i-ELISA was found to show both higher relative sensitivity and specificity than the MRT technique. The percentage of Brucella species detected in milk samples via bacteriological culture was 25.5% and all of them were reported to be B. melitensis biovar 3. On the other hand, 28.3% of samples were positively detected in both conventional and qPCR samples. The relative-sensitivities were 87.10%, 96.77%, and 96.77% for bacteriological culture, conventional-PCR, and qPCR techniques, respectively. All three techniques reported relative-specificities of 100%. The most predominant species detected in cows' milk suffering from reproductive disorders is B. melitensis biovar 3. Both PCR techniques showed the highest relative-sensitivity and specificity in addition to being rapid and low-risk techniques making PCR the best diagnostic technique for brucellosis.

DOI

10.21608/ejvs.2024.259043.1751

Keywords

Milk, Diagnostic Techniques, Relative sensitivity, Relative specificity

Authors

First Name

Nashwa

Last Name

Helmy

MiddleName

M.

Affiliation

Biotechnology Department Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Egypt

Email

nashwahelmy62@yahoo.com

City

-

Orcid

0000-0003-1595-7289

First Name

Hoda

Last Name

Zaki

MiddleName

M.

Affiliation

Bacteriology Department Brucella unit, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Egypt

Email

hodamzaki2010@yahoo.com

City

-

Orcid

-

First Name

Aalaa

Last Name

Saad

MiddleName

-

Affiliation

4) Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. 12611, Giza, Egypt

Email

alaa.samir87@yahoo.com

City

Giza

Orcid

0000-0003-4740-4419

Volume

56

Article Issue

1

Related Issue

46825

Issue Date

2025-01-01

Receive Date

2023-12-28

Publish Date

2025-01-01

Page Start

149

Page End

157

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_351211.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=351211

Order

351,211

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Comparable Study of Immunological, Bacteriological, and Molecular Techniques for Detecting Brucellosis in Milk of Reproductively Problematic Cows

Details

Type

Article

Created At

23 Dec 2024