This study investigate  how gonadotropin injection affects the growth and onset of puberty in Awassi crossbred ewe lambs. Methods: Fourteen Awassi cross breed ewes–lambs of 5±0.5months of age and average body weight (23.7±1.48) kg were divided into two equal groups at random. One group was used as a control, and the other received Gonadotropin Releasing Hormone (GnRH) treatment. Weighing was done on the ewe-lambs both before and after the experiment, Furthermore, measuring the body condition score, number of ewes expressing first estrus after treatment. Samples of blood were taken biweekly until the observation of pubertal signs (ten weeks). Progesterone and insulin-like growth factor-1 (IGF-1) were measured in sera samples. The results revealed that the group receiving gonadotropin reached puberty 17 days ahead of the control group.The body weight and body condition score of the GnRH treated Awassi ewe lambs were found to be higher compared to those of the control group. Gonadotropin administration had a beneficial effect on increasing progesterone and IGF-1. Conclusion Gonadotropin administration has accelerated puberty in Awassi ewe lambs and there are more trophic signals available, as seen by higher serum progesterone and IGF-1 secretions.