THIS STUDY goal was to find out if Spirulina platensis (SP) could help protect rats from the harmful effects of tert-butylhydroquinone (tBHQ). The animals were divided into four groups, each including seven rats. Group 1 consisted of regular rats (-Ve), while group 2 was exposed to an intraperitoneal dose of 100 mg/kg (b.w.) of tBHQ for a period of 7 days (+Ve). Group 3 received a dosage of 500 mg/kg (body weight) of SP and 100 mg/kg of tBHQ, while group 4 was administered a mixture of 750 mg/kg of SP and 100 mg/kg of tBHQ. The rats that were given tBHQ alone had significantly higher of liver enzymes like ALT, AST, and ALP in blood. The tissue levels of the antioxidants glutathione, superoxide dismutase, total antioxidant capacity, and catalase decreased significantly in rats that were exposed to tBHQ-induced toxicity. In the experiment, rats were administered tBHQ as a positive group, leading to an increase in blood lipid profiles (such as TC, TG,) except for HDL-c as compared to the negative treatment. Administration of SP powder led to enhanced blood biochemical parameters and decreased MDA levels in rats who were drunk with tBHQ. Rats exhibited an increase in their antioxidant indicators. The investigation we did shows a strong link between the β-actin, Bcl2, HO-1, and Nrf2 genes in four groups of liver rats that were subjected to experiments. This effect was seen in their blood lipid profile, antioxidant markers, and liver enzymes after exposure to tBHQ.