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347435

Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

This study compares and assesses the efficacy of various hydatid cyst antigens for the accurate immunological diagnosis of CE in both humans and camels. Outer layer (Ol.C) germinal layer (Gl.C) fluid (Fl.C) and protoscolices (Ps.C) antigens prepared from camel hydatid cyst. In addition, fluid (Fl.H) and protoscolices (Ps.H) prepared from human hydatid cyst aspirate. The all six antigen types investigated as immunodiagnostic tools in human and camel hydatidosis using ELISA, SDS-PAGE and western blot assays. ELISA sowed higher binding reactivity by protoscolices antigens (Ps.C and Ps.H) followed by fluid antigens (Fl.C and Fl.H), while outer and germinal layer antigens (Ol.C and Gl.C) give the lower activates. Ps.C antigen exhibiting higher sensitivity (100%), than Ps.H antigen (84.6 and 85.3%) against both positive camels and human sera. The sero-prevalence of Cystic echinococcosis was 59.78% and 43.65% in examined camel and human sera, respectively using the diagnostic Ps.C antigen. According to SDS-PAGE, all six hydatid cyst antigens demonstrated significant electrophoretic similarity, particularly in three bands of 42, 36, and 22 kDa, Using immunoblotting techniques, all separated antigens identified a sharing in two common bands with the same molecular weights of 42 a and 22 kDa. The obtained results concluded that camel and human hydatid cyst protoscolices antigens are introduced in this investigation as an effective diagnostic antigen and new immune-reactive fractions of 42 and 22 kDa for the diagnosis of hydatidosis in humans and camels.

DOI

10.21608/ejvs.2024.275492.1899

Keywords

Hydatid cyst antigens, camel, human, ELISA, SDS and immune blotting

Authors

First Name

Sara

Last Name

Draz

MiddleName

H.

Affiliation

Dept. of Zoonotic Diseases, Veterinary Research Division, National Research Centre

Email

sara_h.adraz@yahoo.com

City

Behira

Orcid

-

First Name

Ahmed

Last Name

Bayoumi

MiddleName

-

Affiliation

Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

Email

ahmedbayoumi603@yahoo.com

City

-

Orcid

-

First Name

Raafat

Last Name

Shaapan

MiddleName

Mohamed

Affiliation

Dept. of Zoonotic Diseases, Veterinary Research Division, National Research Centre

Email

rmshaapan2005@yahoo.com

City

Cairo

Orcid

0000-0002-2620-4189

First Name

Shrif

Last Name

Zidan

MiddleName

A.

Affiliation

Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

Email

shrifzidan@yahoo.com

City

Behira

Orcid

0000-0001-5400-6131

First Name

Nagwa

Last Name

Toaleb

MiddleName

Ibrahim

Affiliation

Prof of Parasitic immunology, Veterinary Research Division

Email

nagwaibrahim26@yahoo.com

City

-

Orcid

0000-0002-9718-5130

First Name

Ghada

Last Name

Hadad

MiddleName

A.

Affiliation

Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

Email

ghadavet@yahoo.com

City

-

Orcid

-

Volume

56

Article Issue

1

Related Issue

46825

Issue Date

2025-01-01

Receive Date

2024-03-08

Publish Date

2025-01-01

Page Start

21

Page End

32

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_347435.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=347435

Order

347,435

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens

Details

Type

Article

Created At

23 Dec 2024