Burns are one of the leading causes of mortality and high morbidity, particularly when combined with bacterial infection that prevents wound closure and delays the healing process. Consequently, there is a huge demand for pharmaceutical techniques to assist in resolving this problem. Essential oils are becoming more popular as organic substitutes for synthetic medications. However, the usage of essential oils is limited due to their poor qualities but they can be utilized by encapsulating them in Nano emulsions. The aim of this study is to investigate the Nano spray formulation containing a combination of lavender, frankincense and peppermint oils for burn healing potential using rat-burn induced model. The effect of Nano emulsions of 2% lavender, 10% peppermint, 5% frankincense, the combination of the three oils in half of their concentrations and the combination in the same concentrations on the fibrotic marker, oxidative stress markers, and histopathological was examined. Our results support the use of essential oils in the development of pharmaceuticals for wounds management and/or inflammatory-related diseases. A trans-dermal Nano spray formulation containing a combination of lavender, frankincense and peppermint oils exhibit considerable wound healing activity.