In this study and through examination of 64 naturally infested stray cats with ticks, the cats were randomly collected from different areas of Mosul city between October 2022 and June 2023. Two species of hard ticks were identified belonging to the genus Rhipicephalus species which are R. sanguineus and R. turanicus. Hematological parameters in the infested cats revealed a significant decrease in PCV, Hb, ESR, RBC and LYMPH which recorded 28.75, 8.75, 7.75, 3435000 and 9.5 respectively. ALP activity showed a very high significant increase and recorded 154 U/L; and a high significant increase in levels AST, LDH and CK activity which recorded 99.53U/L, 605.75U/L and 242.25U/L respectively compared with control groups which recorded 52.8U/L, 266U/L and 77.5 respectively; while ALT level activity showed a significant increase which recorded 41.03U/L compared with control group which recorded 29.4U/L. Significant increased AST, ALT and LDH are also accompanied by an increase in CK. Significant decrease level in Urea, Creatinine, TC and LDL concentration recorded 47.78mmol/L, 1.06µmol/L, 101mg/dL and 45.24 mg/dL compared with control group which recorded 57.76 mmol/L, 1.4µmol/L, 134.5 mg/dL and 65.2 mg/dL respectively. Troponin reported positive. TG and VLDL recorded significant decrease 127.9g/dL and 25.58mg/dL compared with control group which recorded 3.65g/dL, 165.5mg/dL and 33.10 mg/dL respectively. Results showed there are relationships for ALP, LDH and CK activity with a number of parameters measured in infested group in this study.