The most frequently used synthetic sweetener that is used by patients who have high blood glucose and in food administration is sodium saccharin. The danger of sodium saccharin is still in debate, according to the outcomes of some previous research. The aim of this study was to observe the changes that happen due to sodium saccharin's effects on the renal and hepatic organs of laboratory albino rats by histotechnique. Forty experimental rats were used in this study; they were 3–4 months old, and their weight was between 250 and 325 gram. They were separated into four groups (each group contained 10 rats); the control group was the first one (water and food ad libitum). The second, third and fourth groups have been administered by three different measures of sodium saccharin, which were started at 2.5, 5, and 10 mg/kg respectively. The oral gavages were used to give treatments to rats once daily for 120 days; at the end of the experimentation, for histopathological technique, the organs were collected. Depending on the dose, the pathological lesions were different in the hepatic and renal tissues as an outcome of therapeutic effects with sodium saccharin compared to the normal group. The ending results demonstrate that the toxic effects of sodium saccharin in rats taken at 10 mg/kg are higher than those of animals in group 2 treated at 5 mg/kg, and the effects appear lower in group 1 when administered at 2.5 mg/kg of substance. This indicates that sodium saccharin requires an elevated dose to generate pathological changes. The conclusion of this research was that sodium saccharin toxicity became higher for the liver and kidneys by raising its dose.