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Thermal Effect on Ractopamine Residues in Beef and Turkey Meat Using HPLC Assay

Article

Last updated: 20 Jan 2025

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Subjects

-

Tags

Pharmacology & Toxicology

Abstract

Ractopamine (RAC) is commonly used to enhance animal growth and lean body weight, but concerns have been raised about residues in edible tissues and potential adverse effects. Since most food is cooked before consumption, it is important to determine RAC's heat stability in these tissues. Therefore, the main goal of this research was to evaluate the heat stability of RAC in 100 samples of beef and turkey meat using high-pressure liquid chromatography (HPLC) with a UV detector. The results showed that RAC was not detected in any of the turkey muscle samples, while it was found in 22% of the beef muscle samples at levels ranging from 0.5 to 15 ppb, with an average of 6.4. Furthermore, 27.3% of the positive samples exceeded the permissible level. When examining the impact of various thermal methods (boiling, grilling, and frying) on RAC residues, it was observed that boiling, grilling, and frying reduced RAC levels by 17.3%, 42.1%, and 45.4%, respectively. The study revealed that when the cooking temperature increased, the concentration of RAC decreased. The research concluded that RAC degradation varies depending on the degree of cooking temperature, and even after prolonged heating or high temperatures, only half of the remaining RAC in beef muscle was degraded. Finally, cooking methods partially eliminate RAC residues. it is crucial to follow the recommended maximum residue limits for meat samples set by the Codex Alimentarius Commission, as meat is widely consumed that could pose health risks if contaminated with high levels of RAC.

DOI

10.21608/ejvs.2024.276665.1911

Keywords

Ractopamine, β -agonist, Chromatography, meat, cooking

Authors

First Name

Maha,

Last Name

Abd-Elhafeez

MiddleName

S.

Affiliation

Chemistry, Toxicology and Feed Deficiency Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza 12619, Egypt.

Email

mahasabry86_doctor@hotmail.com

City

Giza

Orcid

0000-0002-5201-0214

First Name

Pierre

Last Name

Mehanny

MiddleName

E.

Affiliation

Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt

Email

pezzat7@gmail.com

City

Egypt

Orcid

0009-0000-3282-757X

First Name

Fatma

Last Name

Amro

MiddleName

H.

Affiliation

Food Hygiene Dep., Animal Health Research Institute, Agriculture Research Center, Giza 12618, Egypt.

Email

fatmaamro_87@hotmail.com

City

Giza

Orcid

0000-0001-9193-8528

First Name

Yosra

Last Name

Aleslamboly

MiddleName

S.

Affiliation

Food Hygiene Dep., Animal Health Research Institute, Agriculture Research Center, Giza 12618, Egypt.

Email

yosrasamyy@gmail.com

City

Giza

Orcid

0009-0006-1106-7405

First Name

Eman

Last Name

Elkhawaga

MiddleName

-

Affiliation

Food Hygiene Dept., Animal Health Research Institute, Mansoura, Agricultural Research Center, Egypt

Email

emanelkhawaga@yahoo.com

City

Egypt

Orcid

0009-0007-2818-498X

First Name

Eman

Last Name

Shukry

MiddleName

S.

Affiliation

Food Hygiene Dept., Animal Health Research Institute, Mansoura, Agricultural Research Center, Egypt.

Email

emanshukry81@gmail.com

City

Giza

Orcid

0000-0002-0703-4516

First Name

Azza

Last Name

Abd elmoteleb

MiddleName

Moustafa

Affiliation

Pharmacology and Pyrogen Unit, Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute, Agricultural Research Center, Giza 12618, Egypt.

Email

azzamostafa448@gmail.com

City

Egypt

Orcid

0000-0002-3765-138X

Volume

55

Article Issue

7

Related Issue

46472

Issue Date

2024-12-01

Receive Date

2024-03-13

Publish Date

2024-12-01

Page Start

2,167

Page End

2,176

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 55_Issue 7_Pages 2167-2176.pdf

PDF . 805.7kB

Link

https://ejvs.journals.ekb.eg/article_356790.html

Detail API

http://journals.ekb.eg?_action=service&article_code=356790

Order

36

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Thermal Effect on Ractopamine Residues in Beef and Turkey Meat Using HPLC Assay

Details

Type

Article

Created At

23 Dec 2024