Campylobacter Jejuni is one of the Gram-negative bacteria causing worldwide foodborne illness, especially from poultry species. Human infection may be raised as a consumption of uncooked infected poultry. This study evaluates the occurrence of Campylobacter jejuni in different commercial poultry species (chickens, ducks, and pigeons) and evaluates its virulence. In this study, different organs were collected and pooled together (liver intestine) from chickens, ducks, and pigeons from different Egyptian provinces. The total isolation percentage for Campylobacter species was 26.4% and the highest percentage was from ducks 36% followed by chickens (25%) and pigeons (22.8%), but the majority of Campylobacter Jejuni was detected in Pigeon samples (75%), followed by chickens (60%) and ducks (33.3%). The existence of cdtA, virB11, and aimA. Virulence genes were present in 81%, 33%, and 43% of the total examined C. jejuni strains. This study provides evidence of different virulence mechanisms that have been hidden in different poultry species.