Control of Culex antennatus is an important strategy for eliminating diseases transmition. Application of green-synthesized silver and copper nanoparticles (AgNPs and CuNPs) in mosquito' control has several advantages of eco-friendly because of the absence of deadly chemicals in their synthesis. The present study examined the biological activity of Rosa arabica and Eucalyptus citriodora leaves aqueous extract and its synthesized AgNPs and CuNPs against immature stages of C. antennatus, as well as the reproductive potential of females resulting from treated larvae. It was found that aqueous extract from leaves of R. arabica and E. citriodora can reduce silver and copper ions to generate AgNPs and CuNPs suspended in water. Results of Transmission Electron Microscopy (TEM) evoked the occurrence of AgNPs with sizes ranged between 14.3 and 40.70 nm, while sizes of CuNPs ranged between 19.60 and 69.40 nm, respectively. The UV-vis spectrophotometric analysis for AgNPs and CuNPs revealed the occurrence of single absorption peak at specific wavelengths ranged from 300 to 450 nm, indicating the presence of spherical-shaped NPs. Also, R. arabica and E. citriodora- synthesized AgNPs and CuNPs were more effective against C. antennatus than R. arabica and E. citriodora leaves aqueous extract, however the CuNPs were more effective than AgNPs. The LC₅₀ recorded 38.40 and 31.26 ppm for AgNPs against C. antennatus third larval instar, while CuNPs recorded LC₅₀ of 21.07 and 14.47 ppm, respectively. In addition, synthesized AgNPs and CuNPs significantly reduced the fecundity and fertility of C. antennatus females resulting from treated larvae, as compared with untreated groups.