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343273

Efficiency of MTT and Trypan Blue Assays for Detection of Viability and Recovery of Different Frozen Cell Lines

Article

Last updated: 01 Jan 2025

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Subjects

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Tags

Veterinary Parasitology & Microbiology

Abstract

The quantity of healthy cells in a sample is known as cell viability. For all types of cell culture, measuring cell viability is crucial to ensuring high-quality cell lines through setup, preparation and storage. Although cryopreservation is used in both scientific and clinical research, there are still certain restrictions. At low temperatures, such as −196 °C cells metabolize almost nothing, which has unavoidable side effects, such as genetic drift toward biological variations of cell-associated changes in lipids. Also, cells can be harmed by cryoprotective agents (CPAs), especially when administered in high concentrations So, it is important to use a reliable technique to follow up changes affecting cell viability after cryopreservation which were compared with control freshly subcultured cells. In the present study, the MTT assay was used to correlate cell behavior according to the number of healthy and viable cells. Different kinds of cell cultures were cryopreserved with dimethyl sulfoxide at 10% for variable years in a liquid nitrogen tank . Then, different samples were tested for viability using MTT assay and counted by the trypan blue assay for comparing both results accuracy and confirmed by visual examination in sub-cultured media twenty four hours post-thawing. The MTT assay was easy, fast and showed more accurate results than the trypan blue assay. The visual examination twenty four hours post-subculture indicated that MTT assay can differentiate between healthy and apparently viable cells which have lost their function while the trypan blue assay failed to detect healthy cells in some samples.

DOI

10.21608/ejvs.2024.260687.1764

Keywords

MTTassay, Cell viability, cryopreservation, and trypan blue assay

Authors

First Name

mohamed

Last Name

azzoz

MiddleName

Fahmy

Affiliation

8Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt

Email

mohamed.azzoz.74@gmail.com

City

-

Orcid

-

First Name

mostafa

Last Name

zaghloul

MiddleName

amin

Affiliation

8Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt

Email

moustafazaghloul@gmail.com

City

Giza

Orcid

-

First Name

Maha

Last Name

sayed

MiddleName

Magdy

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt

Email

mahamagdy606@gmail.com

City

Giza

Orcid

-

First Name

heba

Last Name

soliman

MiddleName

mohamed

Affiliation

8Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt

Email

hebasoliman@arc.sci.eg

City

Giza

Orcid

-

First Name

Nermin

Last Name

mounir

MiddleName

mohamed

Affiliation

8Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt

Email

dr_nermeemohamed@yahoo.com

City

Giza

Orcid

-

First Name

Nehal

Last Name

abdel shakour

MiddleName

kamal

Affiliation

6Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Cairo, Egypt.

Email

nahola_kamal@yahoo.com

City

cairo

Orcid

-

First Name

Wahid

Last Name

El-Dabae

MiddleName

Hussein

Affiliation

National research center veterinary research division microbiology and immunology department.

Email

dr_wahidhussein@yahoo.com

City

CAIRO

Orcid

0000-0002-3506-2207

First Name

saleh

Last Name

aly

MiddleName

esmate

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Cairo, Egypt.

Email

salehesmate@gmail.com

City

cairo

Orcid

-

Volume

55

Article Issue

6

Related Issue

45998

Issue Date

2024-11-01

Receive Date

2024-01-04

Publish Date

2024-11-01

Page Start

1,649

Page End

1,657

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 55_Issue 6_Pages 1649-1657.pdf

PDF . 858.7kB

Link

https://ejvs.journals.ekb.eg/article_343273.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=343273

Order

343,273

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Efficiency of MTT and Trypan Blue Assays for Detection of Viability and Recovery of Different Frozen Cell Lines

Details

Type

Article

Created At

23 Dec 2024