The herein study was designed in order to determine the cause of abortion that happened in Al-Zawraa Animal Park and evaluate the healthy and capability of using all mares present in Arabian equine breeding and care center breeding inside Al-Zawraa Zoo Park before starting of breeding season with studying the presence of Streptococcus pyogenes as suspected cause. Vaginal and cervical swabs were taken from twenty-five mares during February 2023 the ages of mares distributed into three pubertal mares were under three years and sixteen mature mares were between four to twenty years as breeding mares and the rest three above twenty-one years as aged female. The routine bacterial isolation and identification of different bacterial type were done and then using PCR technique to detect Streptococcus pyogenes species. Eighteen positive isolations of five different bacteria from vagina, and twelve positive isolations from cervix of four different bacterial types. Streptococcus pyogenes was isolated from mature and aged mares and the aborted mares give positive result of this bacterium in addition to Klebsilla spp. that isolated from mature female only. Those two bacterial species were isolated from both (vaginal and cervical) swabs. We concluded that the cause of abortion in mares was due to infection with Streptococcus pyogenes, meanwhile others two aged female suffer from contamination with Streptococcus pyogenes and Klebsilla spp. which must be treated before using it for breeding. The second conclusion is the use of bacterial evaluation as point of choice before breeding season and subjected of only healthy mares for breeding and treats the infected one.