The current study included isolation, diagnosis, and testing of antibiotic sensitivity for common bacterial agents that cause otitis in dogs. A total of 65 samples of ear swabs were taken from dogs that were presented at the Veterinary Teaching Hospital of the College of Veterinary Medicine/University of Mosul, as well as from private veterinary clinics in Mosul City, between September 2022 and July 2023. Those animals were 1-3 years of age, from both sexes, and they had clinically shown signs of otitis. Upon the bacterial culture properties, the isolated pathogens were as follows: 17 isolates of Pseudomonas aeruginosa (26.1%), 13 isolates of Streptococcus pyogenes (20%), and 10 isolates of Staphylococcus aureus (15.4%). The bacterial isolates were phenotypically identified by Gram stain and growth characteristics on the special culture media. Also, the biochemical properties were identified via the Vitek 2 Compact system. Furthermore, the diagnosis was confirmed by molecular detection of 16S ribosomal RNA (rRNA) of the bacterial agents by using the conventional polymerase chain reaction (PCR). Following that, these bacteria have shown a wide range of susceptibility according to their antibiotic sensitivity test. To conclude, it has been observed that several bacterial pathogens, including P. aeruginosa, S. pyogenes, and S. aureus were the most common causes of bacterial otitis in dogs in Mosul City, and there was a wide range of antimicrobial sensitivity patterns among these microorganisms.