This study was designed to find out the influence of reproductive status, body condition, and localities on hormonal profile, antioxidant status, trace elements, blood biochemical, and metabolites in Egyptian buffaloes. Three experiments were conducted, in experiment 1; buffalo were divided into two groups according to localities (Fayoum & Behira). In experiment 2, 97 buffalo were divided into cyclic (n= 29), early pregnant (n=43), inactive ovaries (n= 12), and endometritis (n= 13). In experiment 3, according to body condition score, buffaloes were divided into three groups (Low, medium, and high). Blood samples were collected from all animals for analyzing progesterone (P₄), estrogen (E₂), superoxide dismutase (SOD), malondialdehyde (MDA), total protein (TP), albumin (Alb), total cholesterol, triglycerides, low- density lipoprotein (LDL), glucose, Zinc (Zn), and copper (Cu). Results indicated that P₄, E₂, antioxidant, TP, Alb, Glob, A/G ratio, glucose, Zn and Cu levels significantly increased (P<0.05) for buffaloes raised at Behira (Delta) compared to Fayoum (Upper Egypt). In pregnant and cyclic buffalo, P₄, E₂, antioxidant, TP, Glob, A/G ratio, cholesterol, triglycerides, glucose, SOD, Zn and Cu increased (P<0.05) in comparison to those with inactive ovaries or endometritis. Moreover, buffaloes with high and medium body conditions had higher (P < 0.05) levels of P₄, E₂, TP, Alb, glob, total cholesterol, triglycerides, and Cu than those having low body condition score. MDA and LDL levels were high (P<0.05) in animals with low body condition score. In conclusion, blood serum concentrations of P₄, E₂, total protein, albumin, globulin, total cholesterol, LDL, triglycerides, Zn, and Cu were the most sensitive biochemical markers to represent the reproductive status of the animals. The body condition score and locality have impacted the fertility of dairy buffalo.