Environmental stresses such as (transportation, stocking density) increase the risk of animals being exposed to infection and injury, affect the efficiency of the immune system, the health status of the animal, and the ability of animals to adapt to these changes. The current study aimed to detected TGF-B and IL-B1 expression associated with microscopic lesions in one day aged broiler chicken. Twenty-eight birds were collected from the Bashiqa district in Mosul city. Results of the current work showed the presences of macro and micro changes, the macro lesions represented by swelling, congestion and hemorrhage spots in different part of the body. In contrast the microscopic exam  of the brain, liver and proventriculus were scored into mild ,moderate and sever lesions which represented by disturbances of growth including  (Atrophy of glandular stomach  and Sloughing of proventriculus folds), disturbance of circulation represented by (edema and congestion), in addition to degeneration and necrosis cases,  immunohistochemistry marker represented by TGF-B and IL-B1 were detected in birds  as a biomarker proteins  for   pathological  samples. These finding suggest that these proteins are the significant proliferative markers in broilers under stress condition and could be used as a prophesy for diagnosis of different disease.