The aim of this study was to find out the correlation between serum leptin, testosterone hormones, and body weight with puberty beginning in male lambs. Eighteen weaned 90day old male lambs, Blood collected to get serum and kept at -20°C for hormonal assay. An ultrasound was used to Get the testicular measurements and measure their echogenicity every 14 days. The results founded that testosterone levels were raised and associated with the age of puberty, and this increase was statistically dissimilar between the following period and each period. The circumference of the testis clearly and significantly increased from day 161 till the examination period finished. Studying the relationship between leptin, testosterone, and testicular size revealed a clear connection between total body weight in pre-puberty and the onset of puberty. Additionally, the testicular circumference and testosterone level were found to have a significant direct association with the Leptin hormone level as the advancing age of puberty. By 174 days of age, the first indications of sexual performance were seen throughout the experimental periods. Moreover, increasing serum leptin levels were seen as puberty progressed. The study finding of male lambs' puberty In pre-pubertal lambs, there was a significant positive connection between the testis parenchyma imaging, moderate echogenicity. and elevated echogenicity in rams that were mature or close to puberty. The study concludes that testosterone levels were statistically raised and associated with the age of puberty with a significant positive association between the pubertal age of Iraqi male lambs at 6 months and the studied leptin, testosterone, and testicular size revealed a clear connection between total body weight which may be useful in determining the pubertal age.