The aim of the study is diagnosis Salmonella infection in broiler chickens in Kirkuk province. For this purpose, 120 samples were collected from broiler chicken organs, which included form the cecal tonsils, liver, spleen, and gallbladder. ELISA, PCR, Culture methods , whole blood agglutination tests, and slide agglutination tests were all used for Salmonella infection diagnosis. The results showed that 52.5% of the broiler chickens had Salmonella bacteria isolated from them, as it was found that the cecal tonsils are the best place to isolate that bacteria, and the results also showed that Salmonella enteritidis is the most prevalent species. The ELISA test resulted 60.3%, , whereas other widely used tests produced a result of 100%. The test of ELISA , the test of slide agglutination and the test of whole blood agglutination for Salmonella enteritidis, with the test of slide agglutination results for Salmonella typhimurium recorded 68.4%,71.9%, 62.3%, and 71.4%, respectively. The study concludes that the best place to isolate the bacteria is the cecal tonsils. In addition, the most common type of Salmonella that was diagnosed in the samples studied was the Salmonella enteritidis.