The study aim to isolates Pseudomonas aeruginosa from urinary tract infections using PCR, virulence gene, and antibiotic resistance detection using conventional and molecular methods. all sample of UIT for detection of P. aeruginosa were diagnosed by conventional ,biochemicals and VITEK-2 Compact technique; each isolate was cultivated on two plates of brain heart infusion agar, one used as a stock culture for antibiotic sensitivity test, while the other was used for genetic materials extraction and amplification of them to detect the presence or not of the virulence genes and the genes responsible for antibiotics resistance. The drug susceptibility test of 24 isolates of Pseudomonas aeruginosa was studied by the classical disk diffusion method against [12] antibiotics on Mueller-Hinton agar.  The percentage ranged between [12.5%] imipenem and [100% ] ampicillin. The molecular confirmation revealed that all 24 isolates were P. aeruginosa and came by the results of the VITEK-2 Compact technique. The results of molecular detection of virulence genes showed that 83.33% [20/24 ] of isolates were positive for the presence of the algD gene, in contrast, 70.83% [17/24] of them proprietor toxA gene. According to the outcomes of molecular identification of antibiotics resistance genes, the overwhelming majority of isolates carried the CTX-M gene [91.66% ], and 75% of them were bearer SHV gene. In comparison, the TEM gene appeared in 45.83% of isolated P. aeruginosa. In conclusion, P. aeruginosa has the genetic weapons for considering and persisting infections, the bacteria own  antibiotic-resistance genes that construct resistant bacteria which makes the cure and control of infection very hard.