Current study was aimed to investigate effect of retained fetal membrane on the concentration of some biochemical parameters and liver enzymes in cows. Total forty five multipara local Iraqi Holstein (n=35) cows suffered from retained fetal membrane were considered as treatment group and 10 normal cows (n=10) as control group, in Salah El-Din province, were used in the current study at the period September 2020 up to April 2021. Blood samples were collected and serum extracted for biochemical parameters and liver enzymes analysis from all animals. The concentration of calcium, phosphorous, iron, magnesium, copper, total serum proteins, glucose, cholesterol and triglyceride were measured by spectrophotometer. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also estimated. The results of present study revealed  that the triglyceride showed significant (P≤0.05) increases in cows suffered from retained fetal membranes (20.863±1.222 mg/dl) compared with healthy cows (16.3±2.172 mg/dl), while the total protein, Glucose and cholesterol didn't show significant differences between groups.  Both Calcium and phosphorous showed significant (P≤0.05) decreases in cows suffered from retained fetal membranes (1.598±0.166 mg/dl and 1.105±0.118 mg/dl respectively) compared with healthy animals (2.058±0.191 mg/dl and 1.52±0.0393 mg/dl respectively). The liver enzymes didn't show any significant changes between the groups.  In conclusion the present study elucidated that calcium and phosphorous are the main minerals which have important role in occurrence of retained fetal membranes in cows.