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Comparison of Growth Hormone Genetic Polymorphism in Awassi Ewes and Their Newborns Using PCR-RFLP Technology

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Animal Reproduction & Artificial Insemination

Abstract

This study was conducted at the University of Mosul / College of Agriculture and Forestry / Department of Animal Production to determine the genetic structures (Genotype) of the Growth hormone gene for Awassi ewes and their progeny and compares between them,  For conducting this work, forty Awassi ewes of age (3-5) years and 20 lambs aged between birth and weaning  (1-3) months were used, The DNA was separated from collected blood samples, growth hormone gene was amplified using specific primers and genotyping was performed using RFLP technique. The distribution ratios of the genetic structures varied in the ewes, as only two genetic structures appeared, namely AA and AG, with a frequency of 0.24 for the AA and 0.16 for the AG genetic structure, and with a allele frequency of (0.8) for the A allele and (0.2) for the G allele, while three genotypes appeared for the lambs, namely AA, AG and GG, with the distribution ratios of the genetic structures  (0.75, 0.1 and 0.15) respectively and with the same allelic frequency (0.8, 0.2) for each of the allele (A and G) respected . We conclude from this study that it is possible to consider the multiple genetic form of the growth hormone gene and its dependence on genetic improvement in ewes and their newborns to achieve a higher economic return than selection methods.

DOI

10.21608/ejvs.2023.237664.1623

Keywords

Growth hormone, PCR-RFLP technology, Float sheep, genotypes

Authors

First Name

Haytham

Last Name

Hady

MiddleName

Mohammed Sabeeh

Affiliation

Department of Animal Production, College of Agriculture and Forestry, University of Mosul, Iraq

Email

haytham-mohammed@uomosul.edu.iq

City

-

Orcid

0000-0001-7570-7201

First Name

Omar

Last Name

Almallah

MiddleName

Dyaa

Affiliation

Department of Animal Production, College of Agriculture and Forestry, University of Mosul, iraq

Email

omaralmallah@uomosul.edu.iq

City

mosul

Orcid

0000-0002-9471-819x

First Name

Falah

Last Name

Al-Hasan

MiddleName

Hasan

Affiliation

Department of Animal Production, College of Agriculture and Forestry, University of Mosul, IRAQ

Email

dr.falah.h@umosul.edu.iq

City

mosul

Orcid

-

Volume

55

Article Issue

4

Related Issue

44526

Issue Date

2024-07-01

Receive Date

2023-09-21

Publish Date

2024-07-01

Page Start

873

Page End

879

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_330100.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=330100

Order

330,100

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Comparison of Growth Hormone Genetic Polymorphism in Awassi Ewes and Their Newborns Using PCR-RFLP Technology

Details

Type

Article

Created At

23 Dec 2024