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325834

Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in Lambs and Calves in Southeastern Anatolia, Turkey

Article

Last updated: 23 Dec 2024

Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

Cryptosporidium spp. and Giardia duodenalis are parasitic protozoa that can infect various hosts, including humans, domestic animals, and wild animals, making them significant from medical and veterinary perspectives. These protozoa are prevalent in cattle and sheep globally, particularly affecting newborn lambs and calves. This study assessed the occurrence and genetic characteristics of Cryptosporidium spp. and G. duodenalis in asymptomatic lambs and calves under three months in Southeast Türkiye. Fecal samples were collected from 124 animals (69 lambs and 55 calves). A direct immunofluorescence test was used to confirm the presence of G. duodenalis cysts and Cryptosporium spp. oocysts. Molecular confirmation was done with Polymerase chain reaction (PCR) using G. duodenalis SSUrRNA and Cryptosporidium spp. SSUrRNA gene. Microscopic analysis of fecal samples, 17.4% and 43.5% of the 69 lambs, 25.5% and 21.8% of the 55 calves in the study were found to be shedding Cryptosporidium spp. and G. duodenalis oocysts/cysts, respectively. Molecular analysis identified Cryptosporidium species as C. parvum, C. xiaoi, and C. ubiquitum in lambs and C. parvum, C. bovis, and C. ryanae in calves. Assemblages A and E from genotype of G. duodenalis were detected in both lambs and calves. The detection of species and genotypes with zoonotic characteristics in asymptomatic lambs and kids concluded that the Cryptosporidium spp. and G. duodenalis oocysts/cysts shed by lams and calves are important for animal and human health.

DOI

10.21608/ejvs.2023.239745.1631

Keywords

calves, Cryptosporidium, Giardia, lamb, SSUrRNA

Authors

First Name

Duygu Neval

Last Name

Sayın İpek

MiddleName

-

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır

Email

dnsayin@hotmail.com

City

-

Orcid

0000-0002-7486-232X

First Name

Barış

Last Name

Sarı

MiddleName

-

Affiliation

Kafkas University, Department of Parazitology, Faculty of Veterinary Medicine

Email

bsari67@gmail.com

City

Kars

Orcid

-

Volume

55

Article Issue

2

Related Issue

43781

Issue Date

2024-03-01

Receive Date

2023-10-02

Publish Date

2024-03-01

Page Start

599

Page End

606

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_325834.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=325834

Order

325,834

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in Lambs and Calves in Southeastern Anatolia, Turkey

Details

Type

Article

Created At

23 Dec 2024