The aim of this study was to elucidate the impact of administering kisspeptin injection on gonadotropin profile (LH) in treated ewes and to evaluate the effect of CIDR and kisspeptin injection on reproductive performance in ewes. Twenty four local Awassi non-pregnant and (3) adult Rams (2-4) years old used in the study. The Experimental animals, were randomly divided into three groups, each consisting of 8 sheep. The First group of control (G1). A second group (G2) Estrus synchronization using CIDR contains 0.3 grams of progesterone for 12 days and at CIDR withdrawal injection of kisspeptin-10 (5µg/kg B.W.) and A third group (G3) Estrus synchronization using CIDR only for 12 days. The investigation encompassed the examination of estrus synchronization and the subsequent natural mating of the animals with proven rams. Blood samples were collected 60 and 30 minutes prior to the administration of kisspeptin and CIDR withdrawal, and 15, 30, 60, 90, 120, 180, 240, 300, and 360 min after kisspeptin injection. Results showed duration of response in G2& G3 was (49.75 and 60.12) respectively and this result is non-significant differences, non-significant differences in conception rate between G2& G3 was (100 % & 66.6 %) respectively, lambing rate was 100% in G2 and 66.6% in G3 and fecundity rate was (137.5%, 83.3%) in G2 and G3 respectively. Regarding the activity of LH, the concentration increase after (15) min of kisspeptin injection. In conclusion, treatment of Awassi ewes with Kisspeptin not effect on reproductive performance and significant affect serum LH concentrations.