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329440

Epidemiological Disclosing and Molecular Subtyping for the Highly Pathogenic Avian Influenza Viruses H5N8 in Commercial Broilers and Layer Chickens in some Egyptian Governorates

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Avian and aquatic Diseases

Abstract

Highly pathogenic avian influenza virus (HPAIV) H5N8 is a serious and fatal respiratory viral disease with an increase in the series of outbreaks in poultry field since its first emergence in Egypt from 2016 till now. Oral and cloacal swabs pool were collected from Sixty domestic poultry flocks of commercial broilers and layer chickens in four Egyptian governorates during 2023 that suffered from classic clinical disease of HPAIV with various mortalities reached 70% during the surveillance period. Molecular analysis was employed to analyze HPAIV subtypes H5 and NA (N1 and N8) by reverse transcriptase polymerase chain reaction (RT-PCR) and further partial hemagglutinin (HA) gene sequencing was carried out also to four positive representative isolates. The results initially revealed the negativity of all tested samples to H5N1 subtype, while H5N8 genome was predominantly positive in 17 out of the 60 examined flocks with prevalence rate 28.3%. Furthermore, the sequencing and phylogenetic tools indicated that our H5N8 isolates were in a close proximity to the clade 2.3.4.4b with the characteristic amino acid motif (PLREKRRKR/GLF) in the HA gene cleavage site which is representative for pathogenic AIV strains. This study concluded the prevalence and predominance of HPAIV H5N8 in the domestic poultry farms in Egypt even in immunized flocks with the strict need of regular monitoring and active surveillances to those circulated strains as well as re-evaluation of the prevention and control protocols and also the vaccination efficacy assessment to overcome such endemic disease.

DOI

10.21608/ejvs.2023.243450.1648

Keywords

Highly pathogenic avian influenza virus, H5N8 subtype, surveillance, Domestic poultry, clade 2.3.4.4b

Authors

First Name

Sameh

Last Name

Amer

MiddleName

Abdel-Moez

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

drsamehnrc@hotmail.com

City

Cairo

Orcid

0000-0002-6017-055X

First Name

Hagar

Last Name

Ahmed

MiddleName

Magdy

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Hassan

MiddleName

Ramadan

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

prof_emy@yahoo.com

City

-

Orcid

-

First Name

Hoda

Last Name

Mekky

MiddleName

Mohamed

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

hmekky335@gmail.com

City

-

Orcid

0000-0002-9808-7065

First Name

Mohamed

Last Name

Bosila

MiddleName

Abd El Rahman

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

mohamed.bosila@yahoo.com

City

-

Orcid

-

First Name

Nagwa

Last Name

Saad Rabie

MiddleName

-

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Khaled

Last Name

El Bayoumi

MiddleName

Mohamed

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Mahmoud Abdel Baki

MiddleName

-

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Asmaa

Last Name

Mahmoud Maatouq

MiddleName

-

Affiliation

Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

Volume

55

Article Issue

3

Related Issue

43446

Issue Date

2024-05-01

Receive Date

2023-10-18

Publish Date

2024-05-01

Page Start

825

Page End

834

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 55_Issue 3_Pages 825-834.pdf

PDF . 1.4MB

Link

https://ejvs.journals.ekb.eg/article_329440.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=329440

Order

329,440

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Epidemiological Disclosing and Molecular Subtyping for the Highly Pathogenic Avian Influenza Viruses H5N8 in Commercial Broilers and Layer Chickens in some Egyptian Governorates

Details

Type

Article

Created At

23 Dec 2024