T
his study examines the action of probiotics (Enterococcus faecium) and prebiotics (glucan and mannan of Saccharomyces cerevisiae cell wall) in the prevention of human colon cancer using the Caco-2 cell line; by measuring the adhesion between probiotics, prebiotics, and Caco-2 cell by plate count agar technique and confocal microscope, estimation of mucin expression by measuring (MUC-2gene), estimation of cytokine expression by measuring interleukin 6, 8, 10 (IL6, IL8, IL10), CXCL1 and tumor necrosis factor (TNF) genes. The confocal microscope indicated the valuable of probiotics and prebiotics in modifying the propagation and apoptosis of cancer cells. The data indicated that the Enterococcus faecium probiotic strain induces elevation on the expression of cxcl1, and IL10 genes and decrease the expression of IL6, IL8, MUC, and TNF genes. While the glucan and mannan of Saccharomyces cerevisiae cell wall induce elevation in the expression of IL6, and MUC genes and decrease the expression of cxcl1, IL8, IL10, and TNF genes. Synbiotic (combination of the Enterococcus faecium and glucan and mannan of Saccharomyces cerevisiae cell wall) induced elevation in the expression of cxcl1, and MUC genes and decrease the expression of IL6, IL8, IL10, and TNF genes. Many research proofs that Enterococcus faecium can be employed as supportive anti-cancer therapies therefore it can protect from cancer