This work aims to fabricate two alternative nanoparticle formulas based on natural materials (Astragalus polysaccharides) as well as metallic materials (gold and silver nanoparticles), which represent quick, simple, and cost-effective approaches for improving the activity of the particles to promote wound healing. The Gold –silver and APS Nanocomposite gels were characterized by using Transmission Electron Microscope (TEM), Zeta potential, dynamic light scattering. The results revealed spherical to sub-spherical shape of the Gold-Silver nanocomposite gel without any aggregation and with homogeneous dispersion in the gel matrix was shown in TEM images . , dynamic light scattering demonstrated that the size were 85 nm and (30-50 nm) for Gold-Silver nanocomposite gel and Astragalus polysaccharides nano gel respectively. The excellent bioactivity of the Gold-Silver nanocomposite gel and the Astragalus polysaccharides nano gel was demonstrated by their respective zeta potential values of -25.2±0.71 mV and +30.4±1.65 mV. The generated nanocomposite gel's capacity for in vivo wound repair was assessed by histopathological confirmation and macroscopical images that showed the healing process. In comparison to Gold-Silver nanocomposite gel and the untreated control group Pharmacological investigations wound size histopathological investigations of the Astragalus polysaccharides treated group revealed significantly greater re-epithelialization, hair follicle regeneration and nearly normal α-SMA . The generated Astragalus polysaccharides nano gel has better wound healing capability when compared to Gold-Silver nanocomposite gel and the untreated groups, according to our results of the investigated pharmacological parameters by increasing the wound closure at the 15th day compared to other groups .