Arsenic (As), a notorious human poison, originates from natural sources. The rising presence of arsenic pollution in groundwater, used for irrigation, human consumption, and industry, has become a major public health concern. This study aimed to assess Spirulina's impact on hematological and lipid parameters in rats exposed to arsenate. Forty-eight female rats were categorized into six groups: a control group with a standard diet, a group receiving 5mg/kg sodium arsenate (As), two groups taking 300mg/kg and 600mg/kg Spirulina (Sp), and two groups combining As and Sp at the same doses. After 28 days, blood samples were collected after an overnight fast and anesthesia for hematological and lipid profile tests. As exposure led to a significant reduction in hemoglobin (Hb) and packed cell volume (PCV) and an increase in white blood cell count (WBCs). However, red blood cell (RBC) parameters, including mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), showed minor non-significant changes. Conversely, treatment with 300mg/kg or 600mg/kg Spirulina resulted in slight increases in RBC parameters, with a notable PCV increase in the 600mg/kg group. Furthermore, As exposure elevated cholesterol, triglyceride, and very low-density lipoprotein (VLDL) levels, while reducing high-density lipoprotein (HDL). However, Sp supplementation at 300mg/kg and 600mg/kg levels restored these lipid parameters to levels similar to the control group, suggesting Spirulina's potential to alleviate arsenic-induced adverse effects on hematological and lipid parameters in rats.