Numerous techniques were used for detoxification of aflatoxin. The present study was conducted to evaluate the addition of mannan oligosaccharides (MOS) and/or Chitosan (Chit) to lessen the toxicity of AFs in broiler chickens (cobb500), improving the performance, biochemical, hematological, antioxidant, and histopathological parameters and gene expression analysis of caspase3 and interleukin 1-β.Five equal groups of 50 broilers each were formed (n=10); negative control (G1) given the basal diet, positive control (G2) basal diet+ 100 ppb AFs kg-1diet; MOS received group (G3) given as basal diet+ 100 ppb AFs kg-1diet + MOS (1 g kg-1 diet); Chit received group (G4) given basal diet+ 100 ppb AFs kg-1diet +Chit (0.5 g kg^-1 diet) ; and MOS/Chit received group (G5) given basal diet+ 100 ppb AFs kg-1diet + MOS (1 g kg^-1 diet)+ Chit (0.5 g kg^-1 diet). All treatments were given to broilers between 6 and 25 days of age. The combination of both MOS and Chit improved hematological parameters in aflatoxicosis-intoxicated groups. Both renal and hepatic markers were significantly improved in chicks intoxicated with aflatoxins and treated with MOS and/or Chit. A significant improvement of antioxidant markers in both MOS and Chit groups exposed to aflatoxicosis. Gene expression of Caspase-3 and IL-1β were significantly improved in chicks intoxicated with aflatoxins and treated with MOS and/or Chit. MOS and/or Chit reduced the levels of accumulation of aflatoxin in muscles, liver, and kidney tissues. The liver sections of the groups (G3, G4, and G5) exhibited significant enhancement in the hepatic parenchymal architecture.