Medicinal plants have shown to be a bountiful wellspring of organically dynamic mixtures for the improvement of new lead synthetic substances for drugs. Screening of Ocimum basilicum and Origanum vulgare plant extricates affirms the presence of different phytochemicals like carbs, cardiovascular glycosides, coumarins, flavonoids, tannins, terpenoid, steroids and phenols in the chose plants. Different phytochemicals as anthraquinone, alkaloids, saponins, proteins and phytosterols were identified and shifted considering the plant constituents. The antioxidant and antimicrobial activities of the Origanum vulgare L. and Ocimum basilicum plant extracts were evaluated by DPPH and by the agar well-diffusion method, respectively. LC-MS/MS analysis of O. basilicum and O. vulgare extracts showed the presence of 14 different compounds. The results confirmed the role of these extracts as promising potent antioxidants and moderate antimicrobial agents. The aim of this investigation was to ascertain the phytochemical screening, the antimicrobial, antioxidant activities and LC-MS/MS analysis of Origanum vulgare and Ocimum basilicum ethanolic extract that obtained from AlBaha in Kingdom of Saudi Arabia.