The purpose of the current investigation was to assess the impact of Tris-extender enriched with a mixture of taurine and different concentrations of Cholesterol-loaded-cyclodextrins( CLC )on preservability of buffalo semen.
Semen was extended in a Tris–citrate glycerol egg yolk extender. Different concentrations of CLC (1.0, 1.5 and2mg mL-1) and fixed concentration of Taurine (60mM) were added into the tubes containing the extended semen in Tris (TCFG) with 20% egg yolk .The control tubes were TCFG containing (zero clc and zero taurine). The straws comprising the control and the experimental extenders were exposed to the freezing process. In Tris taurine Cholesterol-loaded cyclodextrins (TTC) .Extended semen was evaluated initially, post cooling and post freezing. Post cooling sperm motility, Alive sperm , sperm abnormalities and acrosomal percent were kept in all extenders as the control. Sperm membrane integrity (HOST) was considerably superior in all extenders if compared to the control. Post – thawing, sperm motility was significantly higher in TTC1and TTC3. Living spermatozoa was markedly higher in all extenders .Sperm abnormalities were notably inferior in TTC1 . Sperm membrane integrity was extensively improved in all extenders as compared to control. Acrosomal integrity percent was reserved in all extenders relative to control. It could be fulfilled that, in cooled semen, Sperm membrane integrity was significantly higher in all extenders and other Sperm parameters were kept unchanged in comparison with the control. The post – thawed parameters and conception rate exhibited that, the superior was given with the first concentration (TTC1) .