The trauma and damage of the intestinal serosa layer resulted in serious complications. To solute these problems, many substances were used to cover up the affected serosa. This study demonstrated the possibility of using an autogenous peritoneal layer as an implant to overcome the complications of serosal layer damage at the anastomotic area. The study was conducted on twenty-four healthy adult local breed dogs. The serosal layer of the jejunum was removed and then a complete circumferential cut was made in the intestinal wall. The dogs were randomly allocated into two equal groups (n=12 each). In the control group, end-to-end jejunum anastomosis was done by a simple interrupted suturing technique. In the peritoneal group, the area of the anastomosis was sutured as the first group and wrapped by the peritoneum. After 15 days of surgery, both gross and histopathological investigations were studied. Correspondingly, radiographical investigation and bursting pressure test were performed on the 7th and 15th post-surgery. By comparison with the control group, the application of autologous peritoneal graft revealed an efficacious and beneficial effect on the healing process of the intestinal anastomotic site. The healing process in the peritoneal graft group was characterized by rapid mucosal re-epithelization, enhanced collagen fiber formation, and organization, no leakage, reduced adhesions and stenosis, and increased bursting pressure.
In conclusion, wrapping of intestinal anastomosis area with an autologous peritoneal layer after serosal damage is a beneficial and effectual technique without severe complications.