Yersinia enterocolitica (Y. enterocolitica) is a psychrotrophic food-borne pathogen that can cause gastrointestinal disease in humans. Y. enterocolitica is characterized by its capacity to grow at lower degrees and to form biofilm in the food chain. In our study, we aimed to assess the incidence of the planktonic Yersinia enterocolitica strains recovered from poultry meat sources (chicken, duck, geese, and pigeon) in addition to determining their ability to produce biofilm in Egypt. A total of 220 poultry meat samples were gathered randomly from poultry meat and were subjected to conventional culture techniques in order to isolate Y. enterocolitica strains. All the suspected colonies were further examined via uniplex Polymerase Chain Reaction (PCR) using the16S rRNA-specific gene to confirm the Y. enterocolitica suspected isolates. All the confirmed isolates were diagnosed for their potential to form biofilm in vitro using the crystal violet glass tube method. A whole occurrence of Y. enterocolitica was 5.91% (13/220) (. Y. enterocolitica had been recovered from raw chicken meat, raw duck meat, and raw geese meat at rates of 6.67% (10/150), 5% (2/40), and 4.35% (1/23), respectively. A total of nine strains were found to be in biofilm form, while only four strains were a planktonic form. The incidence of Y. enterocolitica in food remains low. However, the planktonic cells were found to have a recovery rate lower than the biofilms which have an industrial and public health concern and must be controlled.