The present study gives an overview of the effect of the administration of warfarin on pregnant rats were exposed orally to warfarin (0.05 mg/kg b. wt. or 0.1mg/kg b. wt.), daily on days zero to 15 of gestation. The pregnant animals were sacrificed on day (20) day of gestation under general anesthesia by a mixture of Xylazine and Ketamine, Collection of blood samples from heart of pregnant rats for biochemical study by using Kit of ELISA technique to estimate the concentration of   Osteopontin.  Fetuses specimens which were embedded by paraffin were sectioned at 4 µm thickness for histopathological and Immunohistochemical techniques were used to compare the relationship of Osteopontin expression to proliferation of intestine and skin in fetuses.
   The result appears Positive expression of SPP1 protein in epidermis layer in fetus skin. and epithelial cells of villi of fetus intestine control group, while the treated rats with low doses of warfarin induced intermediate Expression of SPP1 protein   in epidermis layer in fetus skin and epithelial cells of villi of fetus intestine, also the treated rats with high doses of warfarin   performs weak expression of SPP1 protein in epithelial cells of villi of fetus intestine. It is concluded that Warfarin has negative effect on osteopontin secretion in blood and tissues.