Abstract
Background: Diabetes mellitus, as a chronic metabolic disease, is characterized mainly by hyperglycemia due to insulin deficiency. The cornerstone of diabetes research is the experimental induction of diabetes in laboratory animals, thus various approaches were applied for induction. Objective: The aim of this study was the evaluation of different methods applied to the induction of diabetes in experimental animals. Methods: Alloxan (200 mg/kg of body weight), streptozotocin (50mg/kg body weight) and a high carbohydrate diet were applied in rats, for one month. 45 male albino rats at age 2-4 months and weight (220-260g) were gathered into 5 groups (9 rats for each group). Body physiological parameters and serum glucose, insulin, insulin resistance, and serum lipid profile were estimated. Results: Outcomes reveal that the body weight and body mass index were increased in treated groups, as well as, the greatest serum glucose, minimum serum insulin, and insulin resistance were in the group of the streptozotocin+diet. Whereas the maximum lipid profile fluctuation was in the group of the Alloxan+diet. Conclusion: It could be concluded that the different approaches of experimentally induction of diabetes in rats have a diverse degree of impact on the physiological and biochemical profiles.