This study intended to detect a serological prevalence of avian metapneumovirus (aMPV) antibodies by indirect ELISA. Although its presence in our poultry production is unclear, AMPV is reported as a major poultry virus that causes acute, highly contagious upper respiratory tract infections in chickens and swollen head syndrome, both of which cause considerable cost-effective losses to the chicken industry all over most countries. In the province of Duhok, serum samples totaling 450 were taken from 10, 6-8 weeks old aMPV non vaccinated broiler flocks in the period from November 2021 to March 2022, these flocks will be situated in high poultry farm lands to the east of Duhok. 120 (26.6%) of the 450 serum samples examined by indirect ELISA for the presence of (aMPV) Antibodies, or 4 (40%) of the 10 samples Results from the examination of broiler flocks indicated that this virus infected broiler chickens. Using biosecurity will help reduce infections among broilers, and excellent ventilation practices will help farms as well. This research on an MPV was conducted for the first time in Duhok province, Iraq.