THIS investigation aimed to assess how Pomegranate Seed Extract (PSE) counteracted the negative impacts of Aflatoxin B1 Residue (AFB1) on liver health and antioxidant status in Aflatoxicated Rabbits. Twenty healthy male New Zealand rabbits (aged six weeks) were randomly divided into four experimental groups. Group I served as the negative control group without any treatment. Groups II and III were given AFB1 orally at (30 µg/kg.BW/day) for 21 days. On day one after Aflatoxin B1 administration, groups 3 and 4 were given 100 mg/kg of Pomegranate seed extract (PSE). The results showed that rabbits in groups 3 and 4 given 100 mg/Kg of PSE. Decreased levels of (ALT, AST, cholesterol, triglyceride, and total bilirubin), were observed in Group III which were significantly higher in the treated group II, indicating that PSE supplementation improved liver function tests. In addition, PSE supplementation significantly reduced Malondialdehyde (MDA) levels. Simultaneously, compared to the Aflatoxicated group, there has been a considerable rise in (superoxide dismutase enzyme (SOD), catalase (CAT), glutathione peroxidase (GPX), and reduced glutathione (GSH)) enzymes. According to the findings, Pomegranate seed extract improved treated rabbits' liver function tests and antioxidant status through eliminating the detrimental effects of oxidative stress produced by Aflatoxin B1.