The present study was to estimate the influence of nano-zinc oxide and zinc sulfate on some hematological parameters, liver enzymes, glutathione and malondialdehyde. A total of 108 Rose 308 chicks (1d old) were randomly divided into six groups with three replicates per group (18 birds per group). Treatments included: group one (control with basal diet),group two diet supplemented with nano-zinc oxide 40 mg/kg , group three diet containing zinc sulfate 110 mg/kg , group four subjected to high ambient temperature 40± 2 up to 4 h /day , group five was exposed to high ambient temperature 40± 2 up to 4 h /day and were dietary supplemented with nano-zinc oxide 40 mg/kg , group six was exposed to high ambient temperature 40± 2 up to 4 h /day and were dietary supplemented with zinc sulfate 110 mg/kg. The results showed raise in RBCs and WBC count, Hb concentration, HCT, lymphocytes and heterophiles percentage and glutathione concentration accompanied with decrease in ALT, AST and malondialdehyde concentration in nano-zinc oxide and zinc sulfate compared with control and high ambient temperature. It concluded that nano-zinc oxide and zinc sulfate might improve negative effect of heat stress on some hematological values and liver function in boilers.