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283662

Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing <i> Streptomyces </i> Isolated from Egyptian Localities

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Last updated: 01 Jan 2025

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Abstract

THE AMOUNT of chemosynthetic plastic waste increases every year and the exact time for its degradation is unknown. Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. In the present study, soil samples were collected from the rhizosphere region of seven different crops commonly grown in Egyptian soils and used to collect Streptomyces isolates from Benha, Al-Qalioubia Governorate. A total of 50 representative actinomycete isolates (6 from lettuce, 5 from garlic, 9 from onion, 6 from zucchini, 9 from eggplant, 8 from wheat, 7 from alfalfa rhizosphere soil) were collected and refined. Screening was performed by staining colonies of isolates with Sudan Black B. The dye Sudan Black was used as a screening tool for PHB production. Streptomyces isolates were identified by morphological, biochemical and molecular methods. A phylogenetic tree constructed based on the resulting 16S rRNA-encoding gene sequences was examined. A Streptomyces isolate turned out to be Streptomyces incanus BK128. PHB-purified extract of Streptomyces incanus BK128 was characterized using FTIR in comparison to standard PHB. 

DOI

10.21608/ejrsa.2023.167331.1140

Keywords

PHB, Purified product and SEM, rhizosphere, <i>Streptomyces incanus </i>BK128, 16S rRNA

Authors

First Name

Mahmoud

Last Name

Soliman

MiddleName

A.A.

Affiliation

Department of Radiation Microbiology, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt

Email

actinohallo@yahoo.com

City

-

Orcid

actinohallo@yahoo.com

First Name

Doaa A.

Last Name

Emam

MiddleName

-

Affiliation

Radiation Microbiology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Email

drdoaaemam@gmail.com

City

Cairo

Orcid

-

First Name

Hisham

Last Name

Swailam

MiddleName

M.

Affiliation

Radiation Microbiology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Email

swailamh@yhoo.com

City

Cairo

Orcid

-

First Name

Mhmoud A.

Last Name

Swelim

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Egypt

Email

mhmoud.swil01@fsc.bu.edu.eg

City

-

Orcid

-

First Name

Samar A.

Last Name

Rezk

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Egypt

Email

newfuture84@hotmail.com

City

-

Orcid

-

Volume

35

Article Issue

1

Related Issue

42406

Issue Date

2022-12-01

Receive Date

2022-11-04

Publish Date

2022-12-01

Page Start

69

Page End

81

Print ISSN

1110-0303

Online ISSN

2314-8527

Link

https://ejrsa.journals.ekb.eg/article_283662.html

Detail API

https://ejrsa.journals.ekb.eg/service?article_code=283662

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283,662

Type

Original Article

Type Code

136

Publication Type

Journal

Publication Title

Egyptian Journal of Radiation Sciences and Applications

Publication Link

https://ejrsa.journals.ekb.eg/

MainTitle

Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing <i> Streptomyces </i> Isolated from Egyptian Localities

Details

Type

Article

Created At

23 Dec 2024